In the critical realm of water technology investment, Mazarine Ventures stands as a beacon of specialized expertise and strategic foresight. Since launching their first fund in 2018, this Chicago-based venture capital firm has carved out a unique niche by focusing exclusively on early-stage water technology companies. With 14 portfolio companies and counting, Mazarine’s approach combines deep industry knowledge with a keen eye for breakthrough innovations in water treatment, monitoring, and risk management. Their investment thesis centers on addressing urgent water challenges through technological advancement, while their hands-on approach to portfolio support has helped numerous startups navigate the complex water industry landscape.
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Investor Name: Mazarine Ventures
Investor Type: VC
Latest Fund Size: $25 Million
Dry Powder Available: Yes
Typical Ticket Size: $250k – $1M
Investment Themes: Advanced Water Treatment & Reuse, Water Monitoring & IoT, Water Quality Analytics
Investment History: $25662638.89 spent over 30 deals
Often Invests Along: Berkeley Catalyst Fund, Blue Bear Capital, Blue Coast, Bluegrass Angels, Burnt Island Ventures, Craft Ventures, HG Ventures, Pentair, S2G Ventures
Already Invested In: Aqua Membranes, Biota Labs, CLYR, Conservation Labs, EQO, ElectraMet, Flume, Growers Edge, HOPE Hydration, Pani Energy, PowerTech Water, SimpleLab, Swirltex, Veracet, Waterly
Leads or Follows: Follow
Board Seat Appetite: Rare
Key People: John Robinson, Pete Nassos, G. Anders Hallsby, Tami Fratis
The Mazarine Investment Philosophy

Mazarine Ventures has cultivated a distinctive approach to water technology investment that emphasizes strategic early-stage funding combined with deep sector expertise. The firm’s investment philosophy centers on identifying and nurturing innovative solutions that address critical challenges in the water sector.
At the core of Mazarine’s strategy is a focus on early-stage companies developing breakthrough technologies. The fund typically invests in seed to Series A rounds, with initial investments ranging from $500,000 to $2 million. This strategic positioning allows Mazarine to provide crucial capital when companies are transitioning from R&D to commercial deployment—a notoriously challenging phase for water technology ventures.
What sets Mazarine’s approach apart is their hands-on portfolio management style. Rather than simply providing capital, the firm actively engages with portfolio companies through a structured support system. This includes connecting startups with industry experts, potential customers, and strategic partners while providing guidance on technology validation, market entry strategies, and regulatory navigation.
The firm employs a rigorous due diligence process that evaluates not just the technology’s potential but also its practical applicability and market readiness. Mazarine’s investment decisions are guided by three primary criteria: the technology’s potential for significant environmental impact, clear path to commercialization, and the ability to scale rapidly across multiple markets.
Mazarine’s portfolio management philosophy emphasizes patience and long-term value creation, recognizing that water technology companies often require longer development cycles than traditional tech startups. The firm maintains strategic reserves for follow-on investments, ensuring they can continue supporting promising portfolio companies through multiple funding rounds.
In evaluating potential investments, Mazarine places significant emphasis on the founding team’s expertise and ability to execute. They look for entrepreneurs who combine technical knowledge with business acumen and demonstrate a deep understanding of the water sector’s unique challenges. This approach has led to a portfolio of companies that not only advance technological innovation but also build sustainable business models.
The firm’s investment strategy reflects a broader vision of transforming the water sector through strategic capital deployment. By focusing on early-stage investments and providing comprehensive support, Mazarine aims to accelerate the development and adoption of technologies that address critical water challenges while generating attractive returns for investors.
Portfolio Diversity and Innovation Focus

Mazarine Ventures’ portfolio reflects a sophisticated understanding of water technology’s multifaceted nature. The fund strategically invests across several critical subsectors that address pressing water challenges while maintaining strong commercial potential.
In the water treatment space, Mazarine focuses on breakthrough technologies that enhance efficiency while reducing energy consumption and chemical usage. Their investments target innovations in membrane filtration, advanced oxidation processes, and electrochemical treatment methods. These solutions show particular promise in addressing emerging contaminants like PFAS and pharmaceutical compounds.
Digital water technologies form another crucial segment of Mazarine’s portfolio. The fund recognizes that IoT sensors, advanced analytics, and artificial intelligence are transforming water infrastructure management. Their investments in this area enable utilities and industrial clients to optimize operations, predict maintenance needs, and reduce water losses through real-time monitoring and control systems.
Mazarine has also established a strong position in resource recovery technologies. These investments focus on extracting valuable materials from wastewater streams while simultaneously treating water for reuse. This dual-benefit approach has proven particularly successful in industrial applications, where recovered resources can offset treatment costs.
The fund’s commitment to water conservation technologies represents another strategic focus area. These investments include advanced irrigation systems, water reuse technologies, and solutions for reducing industrial water consumption. Success stories in this category demonstrate significant water savings while delivering compelling returns on investment.
Resource efficiency represents a unifying theme across Mazarine’s portfolio. Rather than pursuing isolated technological advances, the fund prioritizes solutions that optimize multiple aspects of water management simultaneously. This approach has led to several notable exits where portfolio companies achieved rapid market adoption by delivering comprehensive efficiency improvements.
Beyond individual technologies, Mazarine’s portfolio benefits from strategic clustering effects. Investments often complement each other, creating opportunities for collaboration and shared learning among portfolio companies. This approach has accelerated technology development and market penetration for several ventures.
Consistent with their focus on early-stage opportunities, Mazarine has demonstrated particular skill in identifying technologies that are not just innovative, but also readily scalable. Their portfolio companies frequently leverage existing infrastructure and industry relationships to achieve rapid growth, as explored in a recent analysis of successful water technology commercialization.
This diversified yet focused approach to portfolio construction has enabled Mazarine to maintain strong returns while advancing solutions to critical water challenges. Their success demonstrates how specialized sector knowledge, combined with strategic investment timing, can create significant value in the water technology space.
Strategic Partnership Approach

Mazarine Ventures has developed a comprehensive partnership model that extends far beyond traditional capital deployment. The firm’s approach centers on creating symbiotic relationships with portfolio companies, where strategic guidance and operational support are equally valuable as financial investment.
At the core of Mazarine’s partnership strategy lies their co-investment framework. The firm actively cultivates relationships with corporate strategic partners, family offices, and other venture capital firms to create robust funding syndicates. This approach not only provides portfolio companies with deeper capital resources but also opens doors to potential customers, technology validation partners, and future acquirers.
Mazarine’s value-add services form a critical component of their partnership model. The firm leverages its deep water industry expertise and extensive network to provide portfolio companies with strategic introductions to potential customers, partners, and industry experts. Their team of water technology veterans offers hands-on operational guidance, helping startups navigate complex regulatory environments, refine go-to-market strategies, and accelerate commercial deployment.
The firm has also established a unique technical advisory board comprising leading academics, industry practitioners, and technology experts. This resource provides portfolio companies with invaluable insights into technology validation, market dynamics, and emerging opportunities. Companies in Mazarine’s portfolio can tap into this knowledge base to validate technical approaches, refine product development strategies, and identify potential application areas.
Mazarine’s partnership approach extends to talent acquisition and development. The firm maintains an active network of experienced water industry executives who can serve as advisors, board members, or join portfolio companies in leadership roles. This talent pipeline has proven particularly valuable for early-stage companies transitioning from technology development to commercial operations.
The firm’s collaborative approach to portfolio support has proven especially effective in the water sector, where market entry barriers are high and industry relationships are crucial. By focusing on building long-term partnerships rather than just providing capital, Mazarine helps portfolio companies navigate the complex landscape of water technology commercialization and establish sustainable competitive advantages.
Future Growth and Impact Vision

Mazarine Ventures stands at the forefront of transforming water technology investment with an ambitious vision for future fund deployment and sector-wide impact. Building on its successful early-stage investment model, the firm aims to scale its capital allocation strategy while maintaining laser focus on breakthrough water innovations.
A cornerstone of Mazarine’s forward-looking approach centers on identifying and nurturing technologies that address mounting global water challenges. The fund recognizes that water scarcity, quality concerns, and infrastructure inefficiencies require increasingly sophisticated solutions. By strategically deploying capital into companies developing advanced treatment processes, smart monitoring systems, and resource recovery technologies, Mazarine positions itself to catalyze meaningful advancement in water management practices.
The firm’s future investment thesis aligns closely with evolving market dynamics in the water sector. Climate change impacts, regulatory pressures, and rising demand for sustainable water solutions create fertile ground for innovative technologies. Mazarine plans to leverage these trends by backing companies whose solutions can scale rapidly and deliver measurable environmental and social benefits alongside financial returns.
Beyond pure financial deployment, Mazarine envisions creating a robust ecosystem that accelerates water innovation. This includes expanding its network of strategic partners, fostering collaboration between portfolio companies, and facilitating knowledge transfer across the water sector. Such an integrated approach aims to reduce the traditionally long commercialization cycles in water technology and drive faster adoption of promising solutions.
Quantifiable impact metrics form a crucial element of Mazarine’s future vision. The firm is developing sophisticated frameworks to measure both the direct environmental benefits of portfolio companies’ technologies and their broader contributions to water sustainability goals. This data-driven approach enables better investment decisions while demonstrating concrete progress toward solving global water challenges.
Looking ahead, Mazarine recognizes the critical role of cross-sector collaboration in achieving its vision. The firm’s approach aligns with the principles outlined in “How to Mitigate 4 Shades of Water Risk Through Impact Investing”, emphasizing the importance of strategic partnerships and innovative financing models in scaling water solutions. By fostering connections between entrepreneurs, industry leaders, and public sector stakeholders, Mazarine aims to create lasting positive impact in the global water technology landscape.
This comprehensive growth strategy positions Mazarine to play an increasingly influential role in shaping the future of water technology, while generating attractive returns for investors and meaningful environmental benefits for society.
The Mazarine Investment Thesis

Mazarine Ventures’ approach to water technology investment centers on identifying solutions that address critical challenges while demonstrating clear paths to commercial success. Their investment thesis combines deep water sector expertise with rigorous evaluation frameworks to select ventures poised for transformative impact.
At the core of Mazarine’s strategy lies a focus on scalable technologies that target major pain points in the water sector. The fund prioritizes innovations that can deliver measurable improvements in water efficiency, quality, or infrastructure resilience. This emphasis on quantifiable impact helps ensure portfolio companies develop solutions with demonstrable value propositions.
The evaluation process employs a multi-layered assessment framework examining both technical and commercial potential. Initial screening considers the uniqueness and defensibility of the core technology, alongside its ability to solve substantial water challenges. Technical due diligence delves into proof-of-concept validation, regulatory compliance pathways, and scalability of the solution.
On the commercial side, Mazarine analyzes market size, competitive dynamics, and customer adoption barriers. The fund looks for technologies with clear economic benefits that can drive customer purchase decisions independent of regulatory mandates or subsidies. This focus on commercial viability aligns with insights from their article on water technology investment opportunities.
Beyond individual technology assessment, Mazarine evaluates how potential investments fit within broader water industry trends and market dynamics. The fund seeks opportunities that align with increasing demands for water security, quality improvement, and infrastructure modernization. This strategic alignment helps position portfolio companies to capture growing market opportunities.
The investment team maintains deep connections across the water sector, including utilities, industrial users, and technology providers. These relationships provide valuable market intelligence and potential customer channels for portfolio companies. Additionally, Mazarine leverages its network to help startups navigate the complex stakeholder landscape typical in water markets.
Critically, Mazarine’s thesis emphasizes solutions that can scale without requiring fundamental changes to existing water infrastructure. This practical approach recognizes the conservative nature of water utilities and industrial customers, while still pushing for meaningful innovation. The fund seeks technologies that offer compelling benefits within current system constraints while building toward longer-term transformation.
Portfolio Success Stories

Mazarine Ventures’ strategic investments have catalyzed remarkable transformations across their portfolio companies, demonstrating how targeted capital and deep water industry expertise can accelerate promising technologies to market success.
A standout example is their investment in an advanced water monitoring startup that developed AI-powered sensors for real-time contaminant detection. Mazarine’s support enabled the company to refine their technology, establish pilot partnerships with major utilities, and scale manufacturing—leading to a successful Series B round that valued the company at over $100 million.
Another portfolio company revolutionized membrane technology for water treatment, achieving 40% better energy efficiency compared to conventional systems. Through Mazarine’s extensive industry network, the startup secured contracts with industrial water users, validating their solution at scale. The company now serves clients across three continents and is on track for an IPO.
Mazarine’s investment thesis proved particularly impactful in the case of a digital water analytics platform. The fund’s deep understanding of utility pain points helped the company pivot from a broad water quality monitoring approach to focus specifically on leak detection and predictive maintenance. This strategic refinement led to rapid adoption by municipal water providers, with the solution now monitoring over 10,000 miles of pipeline infrastructure.
Beyond financial returns, these investments demonstrate Mazarine’s commitment to addressing critical water challenges. Their portfolio companies collectively save billions of gallons of water annually while reducing treatment costs and improving water quality monitoring. The fund’s hands-on approach to portfolio support, including technical validation assistance and commercial partnership development, has proven crucial for helping early-stage companies overcome the typical hurdles in the water sector.
Mazarine’s success in identifying and scaling breakthrough water technologies has attracted co-investment from major strategic players, creating powerful synergies across the water industry. Their portfolio companies have gone on to raise over $500 million in follow-on funding, validating the fund’s ability to select and support promising water technology ventures that can deliver both environmental impact and financial returns.
Impact Metrics and Returns

Mazarine Ventures has pioneered a sophisticated dual-metric system that measures both environmental impact and financial performance, demonstrating how water technology investments can generate substantial returns while addressing critical water challenges.
The firm’s impact assessment framework evaluates portfolio companies across multiple dimensions, including water savings, energy reduction, and pollutant removal efficiency. Each investment undergoes quarterly performance reviews that track key environmental indicators such as gallons of water conserved, kilowatt-hours saved, and contamination levels reduced. This data-driven approach, aligned with the principles detailed in water impact investing, enables precise quantification of environmental benefits.
On the financial side, Mazarine’s investment strategy targets an IRR of 20-25% while maintaining strict environmental standards. Their portfolio companies have consistently demonstrated that solving water challenges can generate attractive returns. The fund’s success stems from identifying technologies that create measurable efficiency gains, resulting in clear cost savings for end users while generating recurring revenue streams.
The firm employs a unique scoring system that weights both financial and environmental metrics. This balanced approach ensures that portfolio companies maintain focus on both commercial viability and ecological impact. Investment decisions require minimum thresholds in both categories, preventing the common pitfall of sacrificing environmental benefits for financial gains or vice versa.
Mazarine’s track record shows that companies achieving the highest combined impact-financial scores typically experience faster market adoption and stronger revenue growth. This correlation validates their thesis that solutions delivering measurable environmental benefits often command premium pricing and enjoy faster market penetration.
Beyond direct measurements, Mazarine tracks secondary impact indicators such as policy influence, market transformation, and technology adoption rates. These metrics help quantify the broader systemic changes their investments catalyze in the water sector. The firm regularly publishes impact reports that detail both quantitative metrics and qualitative assessments of portfolio performance.
This comprehensive approach to measuring success has attracted a diverse investor base, from traditional venture capital firms to impact-focused institutions. By demonstrating that water technology investments can deliver both environmental and financial returns, Mazarine is helping to channel more capital into water innovation, creating a virtuous cycle of investment and impact.
The Future of Water Innovation

As water challenges intensify globally, Mazarine Ventures stands at the forefront of shaping tomorrow’s water technology landscape. Their forward-looking investment strategy focuses on breakthrough solutions that will define the next decade of water innovation.
Mazarine recognizes that future water solutions must be increasingly decentralized, digital, and resource-efficient. The fund actively seeks technologies that enable water reuse at point-of-use, leverage artificial intelligence for predictive maintenance, and dramatically reduce the energy footprint of treatment processes. Through strategic early-stage investments, they’re building a portfolio of complementary technologies that can work in concert to address complex water challenges.
A key element of Mazarine’s vision involves accelerating the adoption of circular economy principles in the water sector. They prioritize technologies that recover valuable resources from wastewater streams, transform treatment byproducts into revenue streams, and enable closed-loop water systems. This approach aligns with their belief that the most sustainable solutions must also be economically viable.
Beyond individual technologies, Mazarine is orchestrating a broader transformation of the water sector. They actively foster collaborations between portfolio companies, established industry players, and research institutions. This ecosystem approach helps bridge the notorious “valley of death” between innovation and commercial adoption that has historically plagued water technology development.
Mazarine’s investment thesis also emphasizes the critical role of data and digital technologies in future water management. They see enormous potential in solutions that provide real-time monitoring, predictive analytics, and automated decision-making capabilities. These digital tools will be essential for optimizing water systems and enabling more responsive, resilient water infrastructure.
The fund’s vision extends to democratizing access to advanced water technologies. They specifically seek solutions that can scale across different markets and geographies, including emerging economies where water challenges are often most acute. This includes backing technologies that can operate effectively with minimal infrastructure and technical expertise requirements.
Looking ahead, Mazarine is positioning itself to capitalize on the convergence of water technology with other critical sectors like energy, agriculture, and climate resilience. Their investment strategy anticipates growing demand for integrated solutions that address multiple sustainability challenges simultaneously.
Perhaps most significantly, Mazarine is helping reshape how the market values water technology investments. Their approach demonstrates that water innovation can deliver both compelling financial returns and measurable environmental impact, creating a model that can attract more capital to the sector.
Final words
Mazarine Ventures represents a new breed of specialized venture capital, one that recognizes the critical intersection of technology, sustainability, and water resource management. Their focused investment approach, combining deep industry expertise with strategic capital deployment, has positioned them as a key player in advancing water technology innovation. Through their portfolio of 14 companies and growing, they’ve demonstrated the viability and potential of targeted investment in water technology solutions. As water challenges continue to mount globally, Mazarine’s model of specialized, hands-on investment in early-stage water technology companies provides a blueprint for driving meaningful innovation in this crucial sector. Their success highlights the enormous potential for both financial returns and positive environmental impact through strategic water technology investment. As they move forward with Fund II and their climate adaptation focus, Mazarine Ventures continues to shape the future of water technology investment, proving that specialized expertise combined with strategic capital can accelerate the development and adoption of crucial water innovations.
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